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商品属性：
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产品名称
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规格
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货号
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Topoisomerase I (Vaccinia virus)
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100U
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P-PR1235
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Concentration:5U/μl
Storage:-20℃
Description:
Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of
phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right-and
left-handed superhelices. Applications:
Enzyme activity is increased in the presence of 2.5mM Mg2+. Topoisomerase I from vaccinia virus can be used for
studying pivotal biological pr ocess such as-replication, transcription, recombination as well as DNA structure and
topology which includes chromatin reconstitution in vitro and the degree of supercoiling of DNA. Additionally, the
product helps in relaxing the DNA coils and exposes the restriction sites which facilitates in enhancing the restriction
endonuclease digestion of resistant DNA. It is also used for assaying mutant plasmids which differ in length by only one
base-pair. Synonyms:
Topoisomerase I; EC 5.99.1.2; type I DNA topoisomerase; untwisting enzyme;relaxing enzyme; nicking-closing
enzyme; swivelase; ω-protein; deoxyribonucleate topoisomerase; topoisomerase; type I DNA topoisomerase; DNA
topoisomerase; TOPO I
Stroage buffer:
buffered aqueous solution; Solution in 50mM Tris HCl, pH 7.5, containing 100mM NaCl, 1mM EDTA, 1mM DTT, 0.1% Triton X-100, and 50% glycerol. 5×Reaction buffer: 50mM Tris-acetate, pH 7.5, 0.1mM EDTA, 2.5mM MgCl2 and 100mM NaCI. EC Number: EC 5.99.1.2
CAS No.: 80449-01-0
Molecular Weight: mol wt 32kDa
Unit Definition:
One unit coverts 1μg of supercoiled closed circular (Form I) pUC19 DNA to relaxed closed circular form (Form II)
in 1 hr at 37°C.
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